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Omniscript Reverse Transcriptase in first-strand cDNA synthesis high-quality RNA ideal for use in reverse-transcription and RT-PCR applications. Published Examples of Applications for the LightCycler® Carousel-Based Systems. For more information on real-time PCR, see Chapter 7 in this manual. A minus-template PCR, it should have all the PCR components, but use water as template instead of an aliquot of the cDNA (RT reaction). This control will verify.