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In a nutshell, immunohistochemistry uses antibodies to detect proteins (antigens) that are specific to, or have altered expression in, abnormal cells within aImmunohistochemistry (IHC) combines histological, immunological and biochemical techniques for the identification of specific tissue components by means of This brief guide is intended to serve as a reference for researchers to understand, perform, and troubleshoot various IHC protocols during the development and Learn everything from the basics through to advanced techniques and troubleshooting for immunohistochemistry (IHC) with our complete expert guide. Immunohistochemistry (IHC) is a method for studying the localization of antigens in tissue sections using antibodies. Find out more in our guide to IHC. This guide illustrates the basic steps used to create an IHC stain. An Introduction to IHC Staining. IHC is used in histology to detect Learn the steps of immunohistochemistry (IHC) methods from sample preparation to sample labeling and visualization, including options available and reagents A beginners guide to Immunohistochemistry · 1. Tissue preparation · 2. Tissue Preparation: Other things to try · 3. Antigen retrieval · 4. Sample handling · 5.